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細胞分裂周期因子25B(CDC25B)重組蛋白 是CDC25磷酸酶家族的成員。CDC25B通過去除兩個磷酸基團來激活細胞周期蛋白依賴性激酶CDC2,這是進入有絲分裂所必需的。CDC25B由于核定位和核輸出信號在核和細胞質之間穿梭。該蛋白在細胞周期的M和G1期是核蛋白,在S和G2期移動到細胞質。CDC25B具有致癌特性,盡管其在腫瘤形成中的作用尚未確定。
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品牌 | 信裕生物 | CAS | 詳見說明書 |
---|---|---|---|
分子式 | 詳見說明書 | 純度 | > 95% |
分子量 | 23.6kDa | 貨號 | XY90895RPHu |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應用領域 | 生物產業(yè) |
Recombinant Cell Division Cycle Protein 25B (CDC25B)
細胞分裂周期因子25B(CDC25B)重組蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues:Glu377~Gln566
Tags:N-terminal His Tag
Subcellular Location: n/a
Purity: > 95%
Traits:Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Original Concentration:200µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:7.8
Predicted Molecular Mass:23.6kDa
Accurate Molecular Mass:26kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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